Step Dilution & Aliquot Planner
Serial dilution scheme with dead-volume aware aliquoting
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Source
Liquid stock
Solid powder
Stock concentration
M
mM
μM
nM
mg/mL
μg/mL
ng/mL
Mass units need MW only if the target is molar, and vice versa.
Stock volume available
(optional)
L
mL
μL
Measure by
Weight
Amount of substance
Weight
g
mg
μg
ng
Amount
mol
mmol
μmol
nmol
Molar mass (g/mol)
Target
Final concentration
M
mM
μM
nM
mg/mL
μg/mL
ng/mL
pg/mL
Final volume you need
L
mL
μL
Withdrawable volume, after dead volume.
Solvent / diluent
PBS
ddH2O
DMSO
Ethanol
BSA
Methanol
Acetonitrile
Custom
Aliquoting
Tube size (μL)
Dead volume (%)
Share of each tube you cannot pipette back out.
Smallest transfer you trust (μL)
Generate protocol
Copy as markdown
Protocol
Fill in the source and target, then generate a protocol.